TY - JOUR

T1 - FRET-based dynamic structural biology

T2 - Challenges, perspectives and an appeal for open-science practices

AU - Lerner, Eitan

AU - Barth, Anders

AU - Hendrix, Jelle

AU - Ambrose, Benjamin

AU - Birkedal, Victoria

AU - Blanchard, Scott C.

AU - Börner, Richard

AU - Chung, Hoi Sung

AU - Cordes, Thorben

AU - Craggs, Timothy D.

AU - Deniz, Ashok A.

AU - Diao, Jiajia

AU - Fei, Jingyi

AU - Gonzalez, Ruben L.

AU - Gopich, Irina V.

AU - Ha, Taekjip

AU - Hanke, Christian A.

AU - Haran, Gilad

AU - Hatzakis, Nikos S.

AU - Hohng, Sungchul

AU - Hong, Seok Cheol

AU - Hugel, Thorsten

AU - Ingargiola, Antonino

AU - Joo, Chirlmin

AU - Kapanidis, Achillefs N.

AU - Kim, Harold D.

AU - Laurence, Ted

AU - Lee, Nam Ki

AU - Lee, Tae Hee

AU - Lemke, Edward A.

AU - Margeat, Emmanuel

AU - Michaelis, Jens

AU - Michalet, Xavier

AU - Myong, Sua

AU - Nettels, Daniel

AU - Peulen, Thomas Otavio

AU - Ploetz, Evelyn

AU - Razvag, Yair

AU - Robb, Nicole C.

AU - Schuler, Benjamin

AU - Soleimaninejad, Hamid

AU - Tang, Chun

AU - Vafabakhsh, Reza

AU - Lamb, Don C.

AU - Seidel, Claus A.M.

AU - Weiss, Shimon

AU - Boudker, Olga

PY - 2021

Y1 - 2021

N2 - Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever- increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB- Dev. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage ‘open science’ practices.

AB - Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever- increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB- Dev. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage ‘open science’ practices.

U2 - 10.7554/eLife.60416

DO - 10.7554/eLife.60416

M3 - Review

C2 - 33779550

AN - SCOPUS:85102290974

VL - 10

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e60416

ER -