Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles
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A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.
Originalsprog | Engelsk |
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Tidsskrift | Bioconjugate Chemistry |
Vol/bind | 17 |
Udgave nummer | 6 |
Sider (fra-til) | 1373-1375 |
Antal sider | 3 |
ISSN | 1043-1802 |
DOI | |
Status | Udgivet - 2006 |
Eksternt udgivet | Ja |
ID: 286412079