TY - JOUR

T1 - Comparing proteome changes involved in biofilm formation by Streptococcus mutans after exposure to sucrose and starch

AU - Rezaei, Tohid

AU - Kamounah, Fadhil S.

AU - Khodadadi, Ehsaneh

AU - Mehramouz, Bahareh

AU - Gholizadeh, Pourya

AU - Yousefi, Leila

AU - Ganbarov, Khudaverdi

AU - Ghotaslou, Reza

AU - Yousefi, Mehdi

AU - Asgharzadeh, Mohammad

AU - Eslami, Hosein

AU - Taghizadeh, Sepehr

AU - Pirzadeh, Tahereh

AU - Kafil, Hossein Samadi

N1 - Funding Information: This study was supported by Drug Applied Research Center with grant number 67426 and approved by local ethics committee with reference number IR.TBZMED.VCR.REC.1400.187. Publisher Copyright: © 2023 International Union of Biochemistry and Molecular Biology, Inc.

PY - 2023

Y1 - 2023

N2 - Streptococcus mutans is a main organism of tooth infections including tooth decay and periodontitis. The aim of this study was to assess the influence of sucrose and starch on biofilm formation and proteome profile of S. mutans ATCC 35668 strain. The biofilm formation was assessed by microtiter plating method. Changes in bacterial proteins after exposure to sucrose and starch carbohydrates were analyzed using matrix-assisted laser desorption/ionization mass spectrometry. The biofilm formation of S. mutans was increased to 391.76% in 1% sucrose concentration, 165.76% in 1% starch, and 264.27% in the 0.5% sucrose plus 0.5% starch in comparison to biofilm formation in the media without sugars. The abundance of glutamines, adenylate kinase, and 50S ribosomal protein L29 was increased under exposure to sucrose. Upregulation of lactate utilization protein C, 5-hydroxybenzimidazole synthase BzaA, and 50S ribosomal protein L16 was formed under starch exposure. Ribosome-recycling factor, peptide chain release factor 1, and peptide methionine sulfoxide reductase MsrB were upregulated under exposure to sucrose in combination with starch. The results demonstrated that the carbohydrates increase microbial pathogenicity. In addition, sucrose and starch carbohydrates can induce biofilm formation of S. mutans via various mechanisms such as changes in the expression of special proteins.

AB - Streptococcus mutans is a main organism of tooth infections including tooth decay and periodontitis. The aim of this study was to assess the influence of sucrose and starch on biofilm formation and proteome profile of S. mutans ATCC 35668 strain. The biofilm formation was assessed by microtiter plating method. Changes in bacterial proteins after exposure to sucrose and starch carbohydrates were analyzed using matrix-assisted laser desorption/ionization mass spectrometry. The biofilm formation of S. mutans was increased to 391.76% in 1% sucrose concentration, 165.76% in 1% starch, and 264.27% in the 0.5% sucrose plus 0.5% starch in comparison to biofilm formation in the media without sugars. The abundance of glutamines, adenylate kinase, and 50S ribosomal protein L29 was increased under exposure to sucrose. Upregulation of lactate utilization protein C, 5-hydroxybenzimidazole synthase BzaA, and 50S ribosomal protein L16 was formed under starch exposure. Ribosome-recycling factor, peptide chain release factor 1, and peptide methionine sulfoxide reductase MsrB were upregulated under exposure to sucrose in combination with starch. The results demonstrated that the carbohydrates increase microbial pathogenicity. In addition, sucrose and starch carbohydrates can induce biofilm formation of S. mutans via various mechanisms such as changes in the expression of special proteins.

KW - biofilm

KW - carbohydrate

KW - proteome

KW - Streptococcus mutans

U2 - 10.1002/bab.2442

DO - 10.1002/bab.2442

M3 - Journal article

C2 - 36588392

AN - SCOPUS:85145684835

VL - 70

SP - 1320

EP - 1331

JO - Biotechnology and Applied Biochemistry

JF - Biotechnology and Applied Biochemistry

SN - 0885-4513

IS - 3

ER -