The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster
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The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster. / Gambucci, Marta; Cerretani, Cecilia; Latterini, Loredana; Vosch, Tom.
I: Methods and Applications in Fluorescence, Bind 8, Nr. 1, 03.12.2019, s. 014005.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster
AU - Gambucci, Marta
AU - Cerretani, Cecilia
AU - Latterini, Loredana
AU - Vosch, Tom
PY - 2019/12/3
Y1 - 2019/12/3
N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.
AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.
KW - Biosensing Techniques
KW - Chromatography, High Pressure Liquid
KW - DNA/chemistry
KW - Fluorescence
KW - Hydrogen-Ion Concentration
KW - Metal Nanoparticles/chemistry
KW - Osmolar Concentration
KW - Silver/chemistry
KW - Spectrometry, Fluorescence
KW - Time Factors
U2 - 10.1088/2050-6120/ab47f2
DO - 10.1088/2050-6120/ab47f2
M3 - Journal article
C2 - 31794430
VL - 8
SP - 014005
JO - Methods and Applications in Fluorescence
JF - Methods and Applications in Fluorescence
SN - 2050-6120
IS - 1
ER -
ID: 243151498