A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases

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Standard

A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases. / Zhang, Qian; Yang, Mengbi; Sørensen, Kasper K.; Madsen, Charlotte S.; Boesen, Josephine T.; An, Ying; Peng, Shao Hong; Wei, Yuming; Wang, Qianwen; Jensen, Knud J.; Zuo, Zhong; Chan, Ho Yin Edwin; Ngo, Jacky Chi Ki.

I: Scientific Reports, Bind 7, Nr. 1, 12077, 2017.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Zhang, Q, Yang, M, Sørensen, KK, Madsen, CS, Boesen, JT, An, Y, Peng, SH, Wei, Y, Wang, Q, Jensen, KJ, Zuo, Z, Chan, HYE & Ngo, JCK 2017, 'A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases', Scientific Reports, bind 7, nr. 1, 12077. https://doi.org/10.1038/s41598-017-11695-y

APA

Zhang, Q., Yang, M., Sørensen, K. K., Madsen, C. S., Boesen, J. T., An, Y., Peng, S. H., Wei, Y., Wang, Q., Jensen, K. J., Zuo, Z., Chan, H. Y. E., & Ngo, J. C. K. (2017). A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases. Scientific Reports, 7(1), [12077]. https://doi.org/10.1038/s41598-017-11695-y

Vancouver

Zhang Q, Yang M, Sørensen KK, Madsen CS, Boesen JT, An Y o.a. A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases. Scientific Reports. 2017;7(1). 12077. https://doi.org/10.1038/s41598-017-11695-y

Author

Zhang, Qian ; Yang, Mengbi ; Sørensen, Kasper K. ; Madsen, Charlotte S. ; Boesen, Josephine T. ; An, Ying ; Peng, Shao Hong ; Wei, Yuming ; Wang, Qianwen ; Jensen, Knud J. ; Zuo, Zhong ; Chan, Ho Yin Edwin ; Ngo, Jacky Chi Ki. / A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases. I: Scientific Reports. 2017 ; Bind 7, Nr. 1.

Bibtex

@article{b7d39634669b4ade8a612ad10ac0fd00,
title = "A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases",
abstract = "Polyglutamine (PolyQ) diseases are progressive neurodegenerative disorders caused by both protein- and RNA-mediated toxicities. We previously showed that a peptidyl inhibitor, P3, which binds directly to expanded CAG RNA can inhibit RNA-induced nucleolar stress and suppress RNA-induced neurotoxicity. Here we report a N-acetylated and C-amidated derivative of P3, P3V8, that showed a more than 20-fold increase in its affinity for expanded CAG RNA. The P3V8 peptide also more potently alleviated expanded RNA-induced cytotoxicity in vitro, and suppressed polyQ neurodegeneration in Drosophila with no observed toxic effects. Further N-palmitoylation of P3V8 (L1P3V8) not only significantly improved its cellular uptake and stability, but also facilitated its systemic exposure and brain uptake in rats via intranasal administration. Our findings demonstrate that concomitant N-acetylation, C-amidation and palmitoylation of P3 significantly improve both its bioactivity and pharmacological profile. L1P3V8 possesses drug/lead-like properties that can be further developed into a lead inhibitor for the treatment of polyQ diseases.",
author = "Qian Zhang and Mengbi Yang and S{\o}rensen, {Kasper K.} and Madsen, {Charlotte S.} and Boesen, {Josephine T.} and Ying An and Peng, {Shao Hong} and Yuming Wei and Qianwen Wang and Jensen, {Knud J.} and Zhong Zuo and Chan, {Ho Yin Edwin} and Ngo, {Jacky Chi Ki}",
year = "2017",
doi = "10.1038/s41598-017-11695-y",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases

AU - Zhang, Qian

AU - Yang, Mengbi

AU - Sørensen, Kasper K.

AU - Madsen, Charlotte S.

AU - Boesen, Josephine T.

AU - An, Ying

AU - Peng, Shao Hong

AU - Wei, Yuming

AU - Wang, Qianwen

AU - Jensen, Knud J.

AU - Zuo, Zhong

AU - Chan, Ho Yin Edwin

AU - Ngo, Jacky Chi Ki

PY - 2017

Y1 - 2017

N2 - Polyglutamine (PolyQ) diseases are progressive neurodegenerative disorders caused by both protein- and RNA-mediated toxicities. We previously showed that a peptidyl inhibitor, P3, which binds directly to expanded CAG RNA can inhibit RNA-induced nucleolar stress and suppress RNA-induced neurotoxicity. Here we report a N-acetylated and C-amidated derivative of P3, P3V8, that showed a more than 20-fold increase in its affinity for expanded CAG RNA. The P3V8 peptide also more potently alleviated expanded RNA-induced cytotoxicity in vitro, and suppressed polyQ neurodegeneration in Drosophila with no observed toxic effects. Further N-palmitoylation of P3V8 (L1P3V8) not only significantly improved its cellular uptake and stability, but also facilitated its systemic exposure and brain uptake in rats via intranasal administration. Our findings demonstrate that concomitant N-acetylation, C-amidation and palmitoylation of P3 significantly improve both its bioactivity and pharmacological profile. L1P3V8 possesses drug/lead-like properties that can be further developed into a lead inhibitor for the treatment of polyQ diseases.

AB - Polyglutamine (PolyQ) diseases are progressive neurodegenerative disorders caused by both protein- and RNA-mediated toxicities. We previously showed that a peptidyl inhibitor, P3, which binds directly to expanded CAG RNA can inhibit RNA-induced nucleolar stress and suppress RNA-induced neurotoxicity. Here we report a N-acetylated and C-amidated derivative of P3, P3V8, that showed a more than 20-fold increase in its affinity for expanded CAG RNA. The P3V8 peptide also more potently alleviated expanded RNA-induced cytotoxicity in vitro, and suppressed polyQ neurodegeneration in Drosophila with no observed toxic effects. Further N-palmitoylation of P3V8 (L1P3V8) not only significantly improved its cellular uptake and stability, but also facilitated its systemic exposure and brain uptake in rats via intranasal administration. Our findings demonstrate that concomitant N-acetylation, C-amidation and palmitoylation of P3 significantly improve both its bioactivity and pharmacological profile. L1P3V8 possesses drug/lead-like properties that can be further developed into a lead inhibitor for the treatment of polyQ diseases.

U2 - 10.1038/s41598-017-11695-y

DO - 10.1038/s41598-017-11695-y

M3 - Journal article

C2 - 28935901

AN - SCOPUS:85029869568

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 12077

ER -

ID: 184293416