Atomic Structure of a DNA-Stabilized Ag11 Nanocluster with Four Valence Electrons
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Atomic Structure of a DNA-Stabilized Ag11 Nanocluster with Four Valence Electrons. / Rück, Vanessa; Neacșu, Vlad A.; Liisberg, Mikkel B.; Mollerup, Christian B.; Ju, Park Hee; Vosch, Tom; Kondo, Jiro; Cerretani, Cecilia.
I: Advanced Optical Materials, Bind 12, Nr. 7, 2301928, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Atomic Structure of a DNA-Stabilized Ag11 Nanocluster with Four Valence Electrons
AU - Rück, Vanessa
AU - Neacșu, Vlad A.
AU - Liisberg, Mikkel B.
AU - Mollerup, Christian B.
AU - Ju, Park Hee
AU - Vosch, Tom
AU - Kondo, Jiro
AU - Cerretani, Cecilia
N1 - Publisher Copyright: © 2023 The Authors. Advanced Optical Materials published by Wiley-VCH GmbH.
PY - 2024
Y1 - 2024
N2 - The combination of mass spectrometry and single crystal X-ray diffraction of HPLC-purified DNA-stabilized silver nanoclusters (DNA-AgNCs) is a powerful tool to determine the charge and structure of the encapsulated AgNC. Such information is not only relevant to design new DNA-AgNCs with tailored properties, but it is also important for bio-conjugation experiments and is essential for electronic structure calculations. Here, the efforts to determine the structure of a HPLC-purified green emissive DNA-AgNC are presented. Unfortunately, the original DNA-AgNC, known to have four valence electrons, could not be crystallized. By modifying the stabilizing DNA sequence, while maintaining the original spectroscopic properties, several mutants could be successfully crystallized, and for one of them, single crystal X-ray diffraction data provided insight into the silver positions. While the DNA conformation is not resolved, the described approach provides valuable insight into the class of green and dual emissive DNA-AgNCs with four valence electrons. These results constitute a roadmap on how to improve crystallization and crystal quality for X-ray diffraction measurements.
AB - The combination of mass spectrometry and single crystal X-ray diffraction of HPLC-purified DNA-stabilized silver nanoclusters (DNA-AgNCs) is a powerful tool to determine the charge and structure of the encapsulated AgNC. Such information is not only relevant to design new DNA-AgNCs with tailored properties, but it is also important for bio-conjugation experiments and is essential for electronic structure calculations. Here, the efforts to determine the structure of a HPLC-purified green emissive DNA-AgNC are presented. Unfortunately, the original DNA-AgNC, known to have four valence electrons, could not be crystallized. By modifying the stabilizing DNA sequence, while maintaining the original spectroscopic properties, several mutants could be successfully crystallized, and for one of them, single crystal X-ray diffraction data provided insight into the silver positions. While the DNA conformation is not resolved, the described approach provides valuable insight into the class of green and dual emissive DNA-AgNCs with four valence electrons. These results constitute a roadmap on how to improve crystallization and crystal quality for X-ray diffraction measurements.
KW - fluorescence
KW - HPLC purification
KW - mass spectrometry
KW - silver nanoclusters
KW - X-ray diffraction
U2 - 10.1002/adom.202301928
DO - 10.1002/adom.202301928
M3 - Journal article
AN - SCOPUS:85175953101
VL - 12
JO - Advanced Optical Materials
JF - Advanced Optical Materials
SN - 2195-1071
IS - 7
M1 - 2301928
ER -
ID: 373461647