Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry
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Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry. / Rück, Vanessa; Mishra, Narendra K.; Sørensen, Kasper K.; Liisberg, Mikkel B.; Sloth, Ane B.; Cerretani, Cecilia; Mollerup, Christian B.; Kjaer, Andreas; Lou, Chenguang; Jensen, Knud J.; Vosch, Tom.
I: Journal of the American Chemical Society, Bind 145, Nr. 30, 2023, s. 16771–16777.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry
AU - Rück, Vanessa
AU - Mishra, Narendra K.
AU - Sørensen, Kasper K.
AU - Liisberg, Mikkel B.
AU - Sloth, Ane B.
AU - Cerretani, Cecilia
AU - Mollerup, Christian B.
AU - Kjaer, Andreas
AU - Lou, Chenguang
AU - Jensen, Knud J.
AU - Vosch, Tom
N1 - Funding Information: V.R., M.B.L., C.C., and T.V. acknowledge funding from the Villum Foundation (VKR023115) and the Independent Research Fund Denmark (0136-00024B). N.K.M., K.K.S., and K.J.J. thank the Villum Foundation (VKR18333) for funding the Biomolecular Nanoscale Engineering Center (BioNEC), a VILLUM center of excellence, and the Novo Nordisk Foundation funding for the Center for Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery; Grand Challenge Program; NNF16OC0021948). The authors would like to thank Morten Lundh from Gubra for providing the CHO cells expressing the hIR-B. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.
PY - 2023
Y1 - 2023
N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method for linking a well-characterized DNA-AgNC to molecules of interest is presented. Three different peptides and a small protein, human insulin, were tested as labeling targets. The conjugation to the target compounds was verified by MS, HPLC, and time-resolved anisotropy measurements. Moreover, the spectroscopic properties of DNA-AgNCs were found to be unaffected by the linking reactions. For DNA-AgNC-conjugated human insulin, fluorescence imaging studies were performed on Chinese hamster ovary (CHO) cells overexpressing human insulin receptor B (hIR-B). The specific staining of the CHO cell membranes demonstrates that DNA-AgNCs are great candidates for bioimaging applications, and the proposed linking strategy is easy to implement when the DNA-AgNC structure is known.
AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method for linking a well-characterized DNA-AgNC to molecules of interest is presented. Three different peptides and a small protein, human insulin, were tested as labeling targets. The conjugation to the target compounds was verified by MS, HPLC, and time-resolved anisotropy measurements. Moreover, the spectroscopic properties of DNA-AgNCs were found to be unaffected by the linking reactions. For DNA-AgNC-conjugated human insulin, fluorescence imaging studies were performed on Chinese hamster ovary (CHO) cells overexpressing human insulin receptor B (hIR-B). The specific staining of the CHO cell membranes demonstrates that DNA-AgNCs are great candidates for bioimaging applications, and the proposed linking strategy is easy to implement when the DNA-AgNC structure is known.
U2 - 10.1021/jacs.3c04768
DO - 10.1021/jacs.3c04768
M3 - Journal article
C2 - 37441791
AN - SCOPUS:85165644291
VL - 145
SP - 16771
EP - 16777
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 30
ER -
ID: 361438788