Ca2+-Responsive Glyco-insulin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Ca2+-Responsive Glyco-insulin. / Wu, Shunliang; Østergaard, Mads; Fredholt, Freja; Christensen, Niels Johan; Sørensen, Kasper K.; Mishra, Narendra K.; Nielsen, Hanne M.; Jensen, Knud J.

I: Bioconjugate Chemistry, Bind 34, Nr. 3, 2023, s. 518–528.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Wu, S, Østergaard, M, Fredholt, F, Christensen, NJ, Sørensen, KK, Mishra, NK, Nielsen, HM & Jensen, KJ 2023, 'Ca2+-Responsive Glyco-insulin', Bioconjugate Chemistry, bind 34, nr. 3, s. 518–528. https://doi.org/10.1021/acs.bioconjchem.2c00590

APA

Wu, S., Østergaard, M., Fredholt, F., Christensen, N. J., Sørensen, K. K., Mishra, N. K., Nielsen, H. M., & Jensen, K. J. (2023). Ca2+-Responsive Glyco-insulin. Bioconjugate Chemistry, 34(3), 518–528. https://doi.org/10.1021/acs.bioconjchem.2c00590

Vancouver

Wu S, Østergaard M, Fredholt F, Christensen NJ, Sørensen KK, Mishra NK o.a. Ca2+-Responsive Glyco-insulin. Bioconjugate Chemistry. 2023;34(3):518–528. https://doi.org/10.1021/acs.bioconjchem.2c00590

Author

Wu, Shunliang ; Østergaard, Mads ; Fredholt, Freja ; Christensen, Niels Johan ; Sørensen, Kasper K. ; Mishra, Narendra K. ; Nielsen, Hanne M. ; Jensen, Knud J. / Ca2+-Responsive Glyco-insulin. I: Bioconjugate Chemistry. 2023 ; Bind 34, Nr. 3. s. 518–528.

Bibtex

@article{c71bea2dfc364e9c95731c360f4c586e,
title = "Ca2+-Responsive Glyco-insulin",
abstract = "Chemical modification of peptides and proteins, such as PEGylation and lipidation, creates conjugates with new properties. However, they are typically not dynamic or stimuli-responsive. Self-assembly controlled by a stimulus will allow adjusting properties directly. Here, we report that conjugates of oligogalacturonic acids (OGAs), isolated from plant-derived pectin, are Ca2+-responsive. We report the conjugation of OGA to human insulin (HI) to create new glyco-insulins. In addition, we coupled OGA to model peptides. We studied their self-assembly by dynamic light scattering, small-angle X-ray scattering, and circular dichroism, which showed that the self-assembly to form nanostructures depended on the length of the OGA sequence and Zn2+ and Ca2+ concentrations. Subcutaneous administration of OGA12-HI with Zn2+ showed a stable decrease in blood glucose over a longer period of time compared to HI, despite the lower receptor binding affinity.",
author = "Shunliang Wu and Mads {\O}stergaard and Freja Fredholt and Christensen, {Niels Johan} and S{\o}rensen, {Kasper K.} and Mishra, {Narendra K.} and Nielsen, {Hanne M.} and Jensen, {Knud J.}",
note = "Funding Information: We thank the Villum Fonden for funding the Biomolecular Nanoscale Engineering Center (BioNEC), a Villum center of excellence, grant number VKR022710. The Novo Nordisk Foundation is acknowledged for funding the Center of Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery), grant number NNF16OC0021948. We thank Rita Slaaby (PhD) from Novo Nordisk A/S for the receptor affinity study. Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",
year = "2023",
doi = "10.1021/acs.bioconjchem.2c00590",
language = "English",
volume = "34",
pages = "518–528",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "3",

}

RIS

TY - JOUR

T1 - Ca2+-Responsive Glyco-insulin

AU - Wu, Shunliang

AU - Østergaard, Mads

AU - Fredholt, Freja

AU - Christensen, Niels Johan

AU - Sørensen, Kasper K.

AU - Mishra, Narendra K.

AU - Nielsen, Hanne M.

AU - Jensen, Knud J.

N1 - Funding Information: We thank the Villum Fonden for funding the Biomolecular Nanoscale Engineering Center (BioNEC), a Villum center of excellence, grant number VKR022710. The Novo Nordisk Foundation is acknowledged for funding the Center of Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery), grant number NNF16OC0021948. We thank Rita Slaaby (PhD) from Novo Nordisk A/S for the receptor affinity study. Publisher Copyright: © 2023 American Chemical Society.

PY - 2023

Y1 - 2023

N2 - Chemical modification of peptides and proteins, such as PEGylation and lipidation, creates conjugates with new properties. However, they are typically not dynamic or stimuli-responsive. Self-assembly controlled by a stimulus will allow adjusting properties directly. Here, we report that conjugates of oligogalacturonic acids (OGAs), isolated from plant-derived pectin, are Ca2+-responsive. We report the conjugation of OGA to human insulin (HI) to create new glyco-insulins. In addition, we coupled OGA to model peptides. We studied their self-assembly by dynamic light scattering, small-angle X-ray scattering, and circular dichroism, which showed that the self-assembly to form nanostructures depended on the length of the OGA sequence and Zn2+ and Ca2+ concentrations. Subcutaneous administration of OGA12-HI with Zn2+ showed a stable decrease in blood glucose over a longer period of time compared to HI, despite the lower receptor binding affinity.

AB - Chemical modification of peptides and proteins, such as PEGylation and lipidation, creates conjugates with new properties. However, they are typically not dynamic or stimuli-responsive. Self-assembly controlled by a stimulus will allow adjusting properties directly. Here, we report that conjugates of oligogalacturonic acids (OGAs), isolated from plant-derived pectin, are Ca2+-responsive. We report the conjugation of OGA to human insulin (HI) to create new glyco-insulins. In addition, we coupled OGA to model peptides. We studied their self-assembly by dynamic light scattering, small-angle X-ray scattering, and circular dichroism, which showed that the self-assembly to form nanostructures depended on the length of the OGA sequence and Zn2+ and Ca2+ concentrations. Subcutaneous administration of OGA12-HI with Zn2+ showed a stable decrease in blood glucose over a longer period of time compared to HI, despite the lower receptor binding affinity.

U2 - 10.1021/acs.bioconjchem.2c00590

DO - 10.1021/acs.bioconjchem.2c00590

M3 - Journal article

C2 - 36756787

AN - SCOPUS:85147891598

VL - 34

SP - 518

EP - 528

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 3

ER -

ID: 337977003