DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy
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DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy. / Wang, Xiaojin; Liisberg, Mikkel B.; Nolt, Georgia L.; Fu, Xu; Cerretani, Cecilia; Li, Lan; Johnson, Lance A.; Vosch, Tom; Richards, Christopher I.
I: ACS Nano, Bind 17, Nr. 13, 2023, s. 12862–12874.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy
AU - Wang, Xiaojin
AU - Liisberg, Mikkel B.
AU - Nolt, Georgia L.
AU - Fu, Xu
AU - Cerretani, Cecilia
AU - Li, Lan
AU - Johnson, Lance A.
AU - Vosch, Tom
AU - Richards, Christopher I.
PY - 2023
Y1 - 2023
N2 - Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.
AB - Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.
U2 - 10.1021/acsnano.3c04489
DO - 10.1021/acsnano.3c04489
M3 - Journal article
C2 - 37341451
VL - 17
SP - 12862
EP - 12874
JO - A C S Nano
JF - A C S Nano
SN - 1936-0851
IS - 13
ER -
ID: 358639420