Linkers, resins, and general procedures for solid-phase peptide synthesis
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N-alpha-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.
Originalsprog | Engelsk |
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Titel | Peptide synthesis and applications |
Redaktører | Knud J. Jensen, Pernille T. Shelton, Søren L. Pedersen |
Antal sider | 19 |
Forlag | Humana Press |
Publikationsdato | 2013 |
Sider | 23-41 |
ISBN (Trykt) | 978-1-62703-543-9 |
ISBN (Elektronisk) | 978-1-62703-544-6 |
DOI | |
Status | Udgivet - 2013 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1047 |
ISSN | 1064-3745 |
- Solid-phase peptide synthesis, SPPS, TentaGel, ChemMatrix, PEGA, Polystyrene, HBTU, HATU, DIC, COMU, HOBt, HOAt, Oxyma
Forskningsområder
ID: 118886496