Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines. / Janaszewska, Anna; Ciolkowski, Michal; Wróbel, Dominika; Petersen, Johannes Fabritius; Ficker, Mario; Christensen, Jørn Bolstad; Bryszewska, Maria; Klajnert, Barbara.
I: Nanomedicine: Nanotechnology, Biology and Medicine, Bind 9, Nr. 4, 2013, s. 461-464.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines
AU - Janaszewska, Anna
AU - Ciolkowski, Michal
AU - Wróbel, Dominika
AU - Petersen, Johannes Fabritius
AU - Ficker, Mario
AU - Christensen, Jørn Bolstad
AU - Bryszewska, Maria
AU - Klajnert, Barbara
N1 - Copyright © 2013 Elsevier Inc. All rights reserved.
PY - 2013
Y1 - 2013
N2 - Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.
AB - Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.
KW - Animals
KW - Cell Line
KW - Cricetinae
KW - Cricetulus
KW - Dendrimers
KW - Mice
KW - Pyrrolidinones
KW - Rats
U2 - 10.1016/j.nano.2013.01.010
DO - 10.1016/j.nano.2013.01.010
M3 - Journal article
C2 - 23434674
VL - 9
SP - 461
EP - 464
JO - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
SN - 1549-9634
IS - 4
ER -
ID: 95184687