Peptide release, side-chain deprotection, work-up, and isolation
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide Purities and for storage conditions are provided.
Originalsprog | Engelsk |
---|---|
Titel | Peptide synthesis and applications |
Redaktører | Knud J. Jensen, Pernille T. Shelton, Søren L. Pedersen |
Antal sider | 21 |
Forlag | Humana Press |
Publikationsdato | 2013 |
Sider | 43-63 |
ISBN (Trykt) | 978-1-62703-543-9 |
ISBN (Elektronisk) | 978-1-62703-544-6 |
DOI | |
Status | Udgivet - 2013 |
Navn | Methods in Molecular Biology |
---|---|
Vol/bind | 1047 |
ISSN | 1064-3745 |
- Peptide cleavage, Side-chain deprotection, Rink amide linker, Wang linker, Hydrazine benzoyl linker, HMBA linker
Forskningsområder
ID: 118886988