Peptide-stabilized, fluorescent silver nanoclusters: solid-phase synthesis and screening

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Standard

Peptide-stabilized, fluorescent silver nanoclusters : solid-phase synthesis and screening. / Gregersen, Simon; Vosch, Tom André Jos; Jensen, Knud Jørgen.

I: Chemistry: A European Journal, Bind 22, Nr. 51, 2016, s. 18492-18500.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Gregersen, S, Vosch, TAJ & Jensen, KJ 2016, 'Peptide-stabilized, fluorescent silver nanoclusters: solid-phase synthesis and screening', Chemistry: A European Journal, bind 22, nr. 51, s. 18492-18500. https://doi.org/10.1002/chem.201603176

APA

Gregersen, S., Vosch, T. A. J., & Jensen, K. J. (2016). Peptide-stabilized, fluorescent silver nanoclusters: solid-phase synthesis and screening. Chemistry: A European Journal, 22(51), 18492-18500. https://doi.org/10.1002/chem.201603176

Vancouver

Gregersen S, Vosch TAJ, Jensen KJ. Peptide-stabilized, fluorescent silver nanoclusters: solid-phase synthesis and screening. Chemistry: A European Journal. 2016;22(51):18492-18500. https://doi.org/10.1002/chem.201603176

Author

Gregersen, Simon ; Vosch, Tom André Jos ; Jensen, Knud Jørgen. / Peptide-stabilized, fluorescent silver nanoclusters : solid-phase synthesis and screening. I: Chemistry: A European Journal. 2016 ; Bind 22, Nr. 51. s. 18492-18500.

Bibtex

@article{ea52baa0c93543178c22364336672e6e,
title = "Peptide-stabilized, fluorescent silver nanoclusters: solid-phase synthesis and screening",
abstract = "Few-atom silver nanoclusters (AgNCs) can exhibit strong fluorescence; however, they require ligands to prevent aggregation into larger nanoparticles. Fluorescent AgNCs in biopolymer scaffolds have so far mainly been synthesized in solution, and peptides have only found limited use compared to DNA. Herein, we demonstrate how solid-phase methods can increase throughput dramatically in peptide ligand screening and in initial evaluation of fluorescence intensity and chemical stability of peptide-stabilized AgNCs (P-AgNCs). 9-Fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis on a hydroxymethyl-benzoic acid (HMBA) polyethylene glycol polyacrylamide copolymer (PEGA) resin enabled on-resin screening and evaluation of a peptide library, leading to identification of novel peptide-stabilized, fluorescent AgNCs. Using systematic amino acid substitutions, we synthesized and screened a 144-member library. This allowed us to evaluate the effect of length, charge, and Cys content in peptides used as ligands for AgNC stabilization. The results of this study will enable future spectroscopic studies of these peptide-stabilized AgNCs for bioimaging and other applications.",
keywords = "fluorescence, on-resin screening, peptides, silver nanoclusters, solid-phase synthesis",
author = "Simon Gregersen and Vosch, {Tom Andr{\'e} Jos} and Jensen, {Knud J{\o}rgen}",
year = "2016",
doi = "10.1002/chem.201603176",
language = "English",
volume = "22",
pages = "18492--18500",
journal = "Chemistry: A European Journal",
issn = "0947-6539",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "51",

}

RIS

TY - JOUR

T1 - Peptide-stabilized, fluorescent silver nanoclusters

T2 - solid-phase synthesis and screening

AU - Gregersen, Simon

AU - Vosch, Tom André Jos

AU - Jensen, Knud Jørgen

PY - 2016

Y1 - 2016

N2 - Few-atom silver nanoclusters (AgNCs) can exhibit strong fluorescence; however, they require ligands to prevent aggregation into larger nanoparticles. Fluorescent AgNCs in biopolymer scaffolds have so far mainly been synthesized in solution, and peptides have only found limited use compared to DNA. Herein, we demonstrate how solid-phase methods can increase throughput dramatically in peptide ligand screening and in initial evaluation of fluorescence intensity and chemical stability of peptide-stabilized AgNCs (P-AgNCs). 9-Fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis on a hydroxymethyl-benzoic acid (HMBA) polyethylene glycol polyacrylamide copolymer (PEGA) resin enabled on-resin screening and evaluation of a peptide library, leading to identification of novel peptide-stabilized, fluorescent AgNCs. Using systematic amino acid substitutions, we synthesized and screened a 144-member library. This allowed us to evaluate the effect of length, charge, and Cys content in peptides used as ligands for AgNC stabilization. The results of this study will enable future spectroscopic studies of these peptide-stabilized AgNCs for bioimaging and other applications.

AB - Few-atom silver nanoclusters (AgNCs) can exhibit strong fluorescence; however, they require ligands to prevent aggregation into larger nanoparticles. Fluorescent AgNCs in biopolymer scaffolds have so far mainly been synthesized in solution, and peptides have only found limited use compared to DNA. Herein, we demonstrate how solid-phase methods can increase throughput dramatically in peptide ligand screening and in initial evaluation of fluorescence intensity and chemical stability of peptide-stabilized AgNCs (P-AgNCs). 9-Fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis on a hydroxymethyl-benzoic acid (HMBA) polyethylene glycol polyacrylamide copolymer (PEGA) resin enabled on-resin screening and evaluation of a peptide library, leading to identification of novel peptide-stabilized, fluorescent AgNCs. Using systematic amino acid substitutions, we synthesized and screened a 144-member library. This allowed us to evaluate the effect of length, charge, and Cys content in peptides used as ligands for AgNC stabilization. The results of this study will enable future spectroscopic studies of these peptide-stabilized AgNCs for bioimaging and other applications.

KW - fluorescence

KW - on-resin screening

KW - peptides

KW - silver nanoclusters

KW - solid-phase synthesis

U2 - 10.1002/chem.201603176

DO - 10.1002/chem.201603176

M3 - Journal article

C2 - 27809363

AN - SCOPUS:84996490689

VL - 22

SP - 18492

EP - 18500

JO - Chemistry: A European Journal

JF - Chemistry: A European Journal

SN - 0947-6539

IS - 51

ER -

ID: 170741354