Recent advances in covalent, site-specific protein immobilization [version 1; referees: 3 approved]

Publikation: Bidrag til tidsskriftReviewForskningfagfællebedømt

Dokumenter

Morten Peter Meldal, Sanne Schoffelen

The properties of biosensors, biomedical implants, and other materials based on immobilized proteins greatly depend on the method employed to couple the protein molecules to their solid support. Covalent, site-specific immobilization strategies are robust and can provide the level of control that is desired in this kind of application. Recent advances include the use of enzymes, such as sortase A, to couple proteins in a site-specific manner to materials such as microbeads, glass, and hydrogels. Also, self-labeling tags such as the SNAP-tag can be employed. Last but not least, chemical approaches based on bioorthogonal reactions, like the azide–alkyne cycloaddition, have proven to be powerful tools. The lack of comparative studies and quantitative analysis of these immobilization methods hampers the selection process of the optimal strategy for a given application. However, besides immobilization efficiency, the freedom in selecting the site of conjugation and the size of the conjugation tag and the researcher’s expertise regarding molecular biology and/or chemical techniques will be determining factors in this regard
OriginalsprogEngelsk
Artikelnummer2303
TidsskriftF1000Research
Vol/bind5
Antal sider11
ISSN2046-1402
DOI
StatusUdgivet - 2016

Antal downloads er baseret på statistik fra Google Scholar og www.ku.dk


Ingen data tilgængelig

ID: 176611668