WASP integrates substrate topology and cell polarity to guide neutrophil migration

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

WASP integrates substrate topology and cell polarity to guide neutrophil migration. / Brunetti, Rachel M.; Kockelkoren, Gabriele; Raghavan, Preethi; Bell, George R.R.; Britain, Derek; Puri, Natasha; Collins, Sean R.; Leonetti, Manuel D.; Stamou, Dimitrios; Weiner, Orion D.

I: Journal of Cell Biology, Bind 221, Nr. 2, e202104046, 07.02.2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Brunetti, RM, Kockelkoren, G, Raghavan, P, Bell, GRR, Britain, D, Puri, N, Collins, SR, Leonetti, MD, Stamou, D & Weiner, OD 2022, 'WASP integrates substrate topology and cell polarity to guide neutrophil migration', Journal of Cell Biology, bind 221, nr. 2, e202104046. https://doi.org/10.1083/jcb.202104046

APA

Brunetti, R. M., Kockelkoren, G., Raghavan, P., Bell, G. R. R., Britain, D., Puri, N., Collins, S. R., Leonetti, M. D., Stamou, D., & Weiner, O. D. (2022). WASP integrates substrate topology and cell polarity to guide neutrophil migration. Journal of Cell Biology, 221(2), [e202104046]. https://doi.org/10.1083/jcb.202104046

Vancouver

Brunetti RM, Kockelkoren G, Raghavan P, Bell GRR, Britain D, Puri N o.a. WASP integrates substrate topology and cell polarity to guide neutrophil migration. Journal of Cell Biology. 2022 feb. 7;221(2). e202104046. https://doi.org/10.1083/jcb.202104046

Author

Brunetti, Rachel M. ; Kockelkoren, Gabriele ; Raghavan, Preethi ; Bell, George R.R. ; Britain, Derek ; Puri, Natasha ; Collins, Sean R. ; Leonetti, Manuel D. ; Stamou, Dimitrios ; Weiner, Orion D. / WASP integrates substrate topology and cell polarity to guide neutrophil migration. I: Journal of Cell Biology. 2022 ; Bind 221, Nr. 2.

Bibtex

@article{6a52bcaacadb4da191ccbf402e7883b0,
title = "WASP integrates substrate topology and cell polarity to guide neutrophil migration",
abstract = "To control their movement, cells need to coordinate actin assembly with the geometric features of their substrate. Here, we uncover a role for the actin regulator WASP in the 3D migration of neutrophils. We show that WASP responds to substrate topology by enriching to sites of inward, substrate-induced membrane deformation. Superresolution imaging reveals that WASP preferentially enriches to the necks of these substrate-induced invaginations, a distribution that could support substrate pinching. WASP facilitates recruitment of the Arp2/3 complex to these sites, stimulating local actin assembly that couples substrate features with the cytoskeleton. Surprisingly, WASP only enriches to membrane deformations in the front half of the cell, within a permissive zone set by WASP{\textquoteright}s front-biased regulator Cdc42. While WASP KO cells exhibit relatively normal migration on flat substrates, they are defective at topology-directed migration. Our data suggest that WASP integrates substrate topology with cell polarity by selectively polymerizing actin around substrate-induced membrane deformations in the front half of the cell.",
author = "Brunetti, {Rachel M.} and Gabriele Kockelkoren and Preethi Raghavan and Bell, {George R.R.} and Derek Britain and Natasha Puri and Collins, {Sean R.} and Leonetti, {Manuel D.} and Dimitrios Stamou and Weiner, {Orion D.}",
note = "Funding Information: This work was supported by National Institutes of Health grant F31 HL143882 (R.M. Brunetti), National Science Foundation Graduate Research Fellowships Program grant 1650042 (G.R.R. Bell), National Institutes of Health grant GM118167 (O.D. Weiner), National Science Foundation/Biotechnology and Biological Sciences Research Council grant 2019598 (O.D. Weiner), the National Science Foundation Center for Cellular Construction (DBI-1548297), and a Novo Nordisk Foundation grant for the Center for Geometrically Engineered Cellular Systems (NNF17OC0028176). The authors declare no competing financial interests. Publisher Copyright: {\textcopyright} 2021 Brunetti et al.",
year = "2022",
month = feb,
day = "7",
doi = "10.1083/jcb.202104046",
language = "English",
volume = "221",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

RIS

TY - JOUR

T1 - WASP integrates substrate topology and cell polarity to guide neutrophil migration

AU - Brunetti, Rachel M.

AU - Kockelkoren, Gabriele

AU - Raghavan, Preethi

AU - Bell, George R.R.

AU - Britain, Derek

AU - Puri, Natasha

AU - Collins, Sean R.

AU - Leonetti, Manuel D.

AU - Stamou, Dimitrios

AU - Weiner, Orion D.

N1 - Funding Information: This work was supported by National Institutes of Health grant F31 HL143882 (R.M. Brunetti), National Science Foundation Graduate Research Fellowships Program grant 1650042 (G.R.R. Bell), National Institutes of Health grant GM118167 (O.D. Weiner), National Science Foundation/Biotechnology and Biological Sciences Research Council grant 2019598 (O.D. Weiner), the National Science Foundation Center for Cellular Construction (DBI-1548297), and a Novo Nordisk Foundation grant for the Center for Geometrically Engineered Cellular Systems (NNF17OC0028176). The authors declare no competing financial interests. Publisher Copyright: © 2021 Brunetti et al.

PY - 2022/2/7

Y1 - 2022/2/7

N2 - To control their movement, cells need to coordinate actin assembly with the geometric features of their substrate. Here, we uncover a role for the actin regulator WASP in the 3D migration of neutrophils. We show that WASP responds to substrate topology by enriching to sites of inward, substrate-induced membrane deformation. Superresolution imaging reveals that WASP preferentially enriches to the necks of these substrate-induced invaginations, a distribution that could support substrate pinching. WASP facilitates recruitment of the Arp2/3 complex to these sites, stimulating local actin assembly that couples substrate features with the cytoskeleton. Surprisingly, WASP only enriches to membrane deformations in the front half of the cell, within a permissive zone set by WASP’s front-biased regulator Cdc42. While WASP KO cells exhibit relatively normal migration on flat substrates, they are defective at topology-directed migration. Our data suggest that WASP integrates substrate topology with cell polarity by selectively polymerizing actin around substrate-induced membrane deformations in the front half of the cell.

AB - To control their movement, cells need to coordinate actin assembly with the geometric features of their substrate. Here, we uncover a role for the actin regulator WASP in the 3D migration of neutrophils. We show that WASP responds to substrate topology by enriching to sites of inward, substrate-induced membrane deformation. Superresolution imaging reveals that WASP preferentially enriches to the necks of these substrate-induced invaginations, a distribution that could support substrate pinching. WASP facilitates recruitment of the Arp2/3 complex to these sites, stimulating local actin assembly that couples substrate features with the cytoskeleton. Surprisingly, WASP only enriches to membrane deformations in the front half of the cell, within a permissive zone set by WASP’s front-biased regulator Cdc42. While WASP KO cells exhibit relatively normal migration on flat substrates, they are defective at topology-directed migration. Our data suggest that WASP integrates substrate topology with cell polarity by selectively polymerizing actin around substrate-induced membrane deformations in the front half of the cell.

U2 - 10.1083/jcb.202104046

DO - 10.1083/jcb.202104046

M3 - Journal article

C2 - 34964841

AN - SCOPUS:85124680014

VL - 221

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

M1 - e202104046

ER -

ID: 305017330