Cyanohydrin reactions enhance glycolytic oscillations in yeast

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Standard

Cyanohydrin reactions enhance glycolytic oscillations in yeast. / Hald, Bjørn Olav; Nielsen, Astrid Gram; Tortzen, Christian; Sørensen, Preben Graae.

I: Biophysical Chemistry, Bind 200-201, 2015, s. 18-26.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hald, BO, Nielsen, AG, Tortzen, C & Sørensen, PG 2015, 'Cyanohydrin reactions enhance glycolytic oscillations in yeast', Biophysical Chemistry, bind 200-201, s. 18-26. https://doi.org/10.1016/j.bpc.2015.03.004

APA

Hald, B. O., Nielsen, A. G., Tortzen, C., & Sørensen, P. G. (2015). Cyanohydrin reactions enhance glycolytic oscillations in yeast. Biophysical Chemistry, 200-201, 18-26. https://doi.org/10.1016/j.bpc.2015.03.004

Vancouver

Hald BO, Nielsen AG, Tortzen C, Sørensen PG. Cyanohydrin reactions enhance glycolytic oscillations in yeast. Biophysical Chemistry. 2015;200-201:18-26. https://doi.org/10.1016/j.bpc.2015.03.004

Author

Hald, Bjørn Olav ; Nielsen, Astrid Gram ; Tortzen, Christian ; Sørensen, Preben Graae. / Cyanohydrin reactions enhance glycolytic oscillations in yeast. I: Biophysical Chemistry. 2015 ; Bind 200-201. s. 18-26.

Bibtex

@article{abe5d42971934fe7a01787b060a7be36,

title = "Cyanohydrin reactions enhance glycolytic oscillations in yeast",

abstract = "Synchronous metabolic oscillations can be induced in yeast by addition of glucose and removal of extracellular acetaldehyde (ACAx). Compared to other means of ACAx removal, cyanide robustly induces oscillations, indicating additional cyanide reactions besides ACA to lactonitrile conversion. Here, (13)C NMR is used to confirm our previous hypothesis, that cyanide directly affects glycolytic fluxes through reaction with carbonyl-containing compounds. Intracellularly, at least 3 cyanohydrins were identified. Extracellularly, all signals could be identified and lactonitrile was found to account for ~66% of total cyanide removal. Simulations of our updated computational model show that intracellular cyanide reactions increase the amplitude of oscillations and that cyanide addition lowers [ACA] instantaneously. We conclude that cyanide provides the following means of inducing global oscillations: a) by reducing [ACAx] relative to oscillation amplitude, b) by targeting multiple intracellular carbonyl compounds during fermentation, and c) by acting as a phase resetting stimulus.",

author = "Hald, {Bj{\o}rn Olav} and Nielsen, {Astrid Gram} and Christian Tortzen and S{\o}rensen, {Preben Graae}",

year = "2015",

doi = "10.1016/j.bpc.2015.03.004",

language = "English",

volume = "200-201",

pages = "18--26",

journal = "Biophysical Chemistry",

issn = "0301-4622",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Cyanohydrin reactions enhance glycolytic oscillations in yeast

AU - Hald, Bjørn Olav

AU - Nielsen, Astrid Gram

AU - Tortzen, Christian

AU - Sørensen, Preben Graae

PY - 2015

Y1 - 2015

N2 - Synchronous metabolic oscillations can be induced in yeast by addition of glucose and removal of extracellular acetaldehyde (ACAx). Compared to other means of ACAx removal, cyanide robustly induces oscillations, indicating additional cyanide reactions besides ACA to lactonitrile conversion. Here, (13)C NMR is used to confirm our previous hypothesis, that cyanide directly affects glycolytic fluxes through reaction with carbonyl-containing compounds. Intracellularly, at least 3 cyanohydrins were identified. Extracellularly, all signals could be identified and lactonitrile was found to account for ~66% of total cyanide removal. Simulations of our updated computational model show that intracellular cyanide reactions increase the amplitude of oscillations and that cyanide addition lowers [ACA] instantaneously. We conclude that cyanide provides the following means of inducing global oscillations: a) by reducing [ACAx] relative to oscillation amplitude, b) by targeting multiple intracellular carbonyl compounds during fermentation, and c) by acting as a phase resetting stimulus.

AB - Synchronous metabolic oscillations can be induced in yeast by addition of glucose and removal of extracellular acetaldehyde (ACAx). Compared to other means of ACAx removal, cyanide robustly induces oscillations, indicating additional cyanide reactions besides ACA to lactonitrile conversion. Here, (13)C NMR is used to confirm our previous hypothesis, that cyanide directly affects glycolytic fluxes through reaction with carbonyl-containing compounds. Intracellularly, at least 3 cyanohydrins were identified. Extracellularly, all signals could be identified and lactonitrile was found to account for ~66% of total cyanide removal. Simulations of our updated computational model show that intracellular cyanide reactions increase the amplitude of oscillations and that cyanide addition lowers [ACA] instantaneously. We conclude that cyanide provides the following means of inducing global oscillations: a) by reducing [ACAx] relative to oscillation amplitude, b) by targeting multiple intracellular carbonyl compounds during fermentation, and c) by acting as a phase resetting stimulus.

U2 - 10.1016/j.bpc.2015.03.004

DO - 10.1016/j.bpc.2015.03.004

M3 - Journal article

C2 - 25863195

VL - 200-201

SP - 18

EP - 26

JO - Biophysical Chemistry

JF - Biophysical Chemistry

SN - 0301-4622

ER -

ID: 135658618

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