Sandwich type plasmonic platform for MEF using silver fractals
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Sandwich type plasmonic platform for MEF using silver fractals. / Raut, Sangram L.; Rich, Ryan; Shtoyko, Tanya; Bora, Ilkay; Laursen, Bo Wegge; Sørensen, Thomas Just; Borejdo, Julian; Gryczynski, Zygmunt; Gryczynski, Ignacy.
I: Nanoscale, Bind 7, Nr. 42, 2015, s. 17729-17734.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Sandwich type plasmonic platform for MEF using silver fractals
AU - Raut, Sangram L.
AU - Rich, Ryan
AU - Shtoyko, Tanya
AU - Bora, Ilkay
AU - Laursen, Bo Wegge
AU - Sørensen, Thomas Just
AU - Borejdo, Julian
AU - Gryczynski, Zygmunt
AU - Gryczynski, Ignacy
PY - 2015
Y1 - 2015
N2 - In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA·Cl) in PVA films made from 0.2% PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) were assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA·Cl in PVA film. In addition, we also tested ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than a 20-fold fluorescence enhancement (bright spots) accompanied by a decrease in the fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with or without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.
AB - In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA·Cl) in PVA films made from 0.2% PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) were assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA·Cl in PVA film. In addition, we also tested ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than a 20-fold fluorescence enhancement (bright spots) accompanied by a decrease in the fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with or without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.
U2 - 10.1039/c5nr05834a
DO - 10.1039/c5nr05834a
M3 - Journal article
C2 - 26452215
VL - 7
SP - 17729
EP - 17734
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
IS - 42
ER -
ID: 147971814